Primer

Part:BBa_K391003:Design

Designed by: Phillip Chu   Group: iGEM10_British_Columbia   (2010-10-25)


Reverse Primer to PCR agrCA (with RBS) from S. aureus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

These primers flank naturally occuring RBS sites for agrCA. agrC is upstream of agrA in the genome. The PCR starts approximately 100 bp of agrC at a putative RBS site, ensuring that a RBS site is present. agrA, because of its location, will have a RBS upstream as well.


Source

From S. aureus strain NCTC 8325. Accession number: NC_007795. Available on NCBI.


References